Coding

Part:BBa_K5403002:Design

Designed by: Lizette van der Ziel   Group: iGEM24_TU-Eindhoven   (2024-08-27)


Flexible GSGSGSGSAS linker with NheI restriction site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This linker was designed to provide flexibility when fusing two proteins together recombinantly. The glycine and serine residues allow for rotation, causing many possible confirmations.

This part is meant to be fused to the N-terminal protein of the fusion protein, on its C-terminal end. The new C-terminal end then has an NheI restriction site with which the C-terminal protein of the fusion protein can be attached.

The codons in this linker were chosen based on (1) the codon prevalence within m. smegmatis genetics and (2) the GC-content. The most common codons in m. smegmatis DNA are high in GC-content, which complicates the artificial synthesis of these genes. Therefore, the second most common codon was chosen if it had a lower GC-content. More concretely, this meant that for glycines, GGT was used instead of GGC/GGG.

Examples of parts that use this GS-linker are BBa_K5403012 - BBa_K5403020.